Table 5. Detailed PCR findings for patients with acute Chagas disease.

Patient ID	patient description	time of specimen	specimen type	results individual assays			Reported results
				kDNA TaqMan	TCZ TaqMan	18 S rRNA TaqMan
13	acute infection after triatomine contact in endemic region1	sample 1	EDTA blood	positive	positive	positive	positive
		1 week later	blood clot	positive	positive	positive	positive †
		3 weeks later	EDTA blood	positive	positive	positive	positive †
		2 months later	EDTA blood	negative	negative	negative	negative†
		3 months later	EDTA blood	negative	negative	negative	negative
		4 months later	EDTA blood	positive	positive	negative	positive
		4.5 months later	EDTA blood	positive	negative	negative	equivocal
14	congenital transmission2	19 days old	EDTA blood	positive	positive	positive	positive
		2 months old	EDTA blood	negative	negative	negative	negative†

¹Patient was serologically positive for Chagas disease by the time she was tested at CDC.

²Patient was serologically positive when 19 days old due to maternal antibodies. Another sample collected at 10 months of age tested negative in serology.

†: indicate specimens that were collected during drug treatment (benznidazole or nifurtimox).