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. 2012 Jul 3;6(7):e1680. doi: 10.1371/journal.pntd.0001680

Figure 5. K11777 targets cysteine protease activity in A. ceylanicum in vivo.

Figure 5

Soluble extracts of hookworms taken from hamsters 8 h post-treatment with single doses of ABZ (10 mg/kg), K11777 (100 mg/kg) or vehicle alone were prepared. Specific cysteine protease activity (relative fluorescence units/min/µg extract) was measured in 0.05 M sodium acetate, 2 mM DTT, pH 5.5 containing 10 µM of the dipeptidyl fluorogenic substrate Z-Phe-Arg-AMC. All activity could be inhibited by 1 µM K11777 (not shown) indicating that only cysteine protease activity was being measured. Data points are expressed as means ± SD of three separate experiments each performed in duplicate. Values for CP activity between the three experimental groups were statistically different using paired t-tests: P = 0.0024 for vehicle vs. K11777; P = 0.0074 for ABZ vs. K11777 and P = 0.0054 for vehicle vs. ABZ.