(A) Bone marrow cells from Ubp43+/+ and Ubp43−/− mice were cultured in the absence or presence of IFN-β (500 units/ml) for 24 hrs (top panel for caspase-3 detection) or for 6 hrs (bottom panel for Bid detection). caspase-3-FL, full-length form of caspase-3; caspase-3-CL, activated cleaved form of caspase-3. (B) THP-1 cells stably expressing shControl or UBP43-specific shRNA#1 were treated with IFN-α as in Fig. 1B. The cells were harvested after the 2nd treatment of IFN-α at various time points and analyzed by immunoblotting. (C and D) Cultured bone marrow cells from Ubp43+/+ and Ubp43−/− mice (C) or THP-1 cells stably expressing shControl or UBP43-specific shRNA#1 (D) were treated with TRAIL (0, 300, or 500 ng/ml) or FASL (0, 100, or 300 ng/ml) for 24 hrs, and analyzed for apoptosis.