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. Author manuscript; available in PMC: 2013 Jun 5.
Published in final edited form as: Anal Chem. 2012 May 24;84(11):4659–4664. doi: 10.1021/ac301203m

Figure 2.

Figure 2

Analysis of interference of acetylcholine measurement using LC-ESI-MS/MS. (A) Analysis of dialysate samples: (i) MRM (146→87) trace for acetylcholine (ACh) showing interference; (ii) MRM (150→91) trace for d4-acetylcholine. (B) Determination of source of acetylcholine interference and its reduction. (i) LC-ESI-MS/MS (150→91) trace of 1 μM d4-acetylcholine in aCSF; (ii) LC-ESI-MS/MS (146→87) trace of aCSF without MgSO4 showing no interfering peak; (iii) LC-ESI-MS/MS (146→87) trace of regular aCSF blank solution showing interference; (iv) LC-ESI-MS/MS (146→87) trace of regular aCSF blank spiked with 3 mM EDTA showing reduction of interfering peak.