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Quantitative performance of droplet-microdialysis-ESI-MS acetylcholine detection set up. (A) Calibration curve as ratio of signal for acetylcholine to internal standard (I.S.) Standard was prepared in aCSF. (B) Trace of different concentrations of acetylcholine by segmented flow ESI-MS/MS. Inset shows enlarged view of 16 samples at 100 nM. Result was obtained by sampling from different concentration acetylcholine standard solutions using the system shown in Figure 1.
