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. Author manuscript; available in PMC: 2013 Jun 5.
Published in final edited form as: Anal Chem. 2012 May 24;84(11):4659–4664. doi: 10.1021/ac301203m

Figure 5.

Figure 5

Recording of response to in vivo microinjection of neostigmine. Figure showed a sample trace for simultaneous detection of (A) acetylcholine (ACh); (B) internal standard, d4-acetylcholine (d4-ACh); and (C) neostigmine (Neo). Arrow indicates beginning of each microinjection. Insert is a magnified view of the 1st microinjection for neostigmine detection. (D) Averaged acetylcholine response to neostigmine microinjection (dotted line) overlaid with averaged neostigmine signal (solid line), (n = 6, error bar = SEM). Response of 2 to 3 droplets were binned in to one data point at 10 s interval for the averaged plot.