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Analyte MRM conditions. Three analytes were monitored together during in vivo experiments: acetylcholine, d4-acetylcholine, and choline or acetylcholine, d4-acetylcholine and neostigmine. Total MS cycle time was ~ 0.2 s.
| analyte | transition | dwell time (ms) | cone voltage | collision energy |
|---|---|---|---|---|
| acetylcholine | 146→87 | 70 | 35 | 15 |
| d4-acetylcholine | 150→91 | 70 | 35 | 15 |
| choline | 104→60 | 70 | 35 | 15 |
| neostigmine | 223→208 | 70 | 35 | 20 |
