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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Differentiation. 2012 May 11;84(1):103–116. doi: 10.1016/j.diff.2012.04.001

Figure 2. Expression of Transglutaminase-2 (TG2) Defines a Cushion Boundary Interface.

Figure 2

Immunohistochemical analyses for TG2 were performed on chick embryos through embryonic and fetal life. Expression of this enzyme at HH24 is restricted to a region close to the myocardium (bracket), whereas the distal half of the cushion is devoid of expression. Expression following cushion fusion (HH27) and during maturation (HH35) demonstrates nearly all interstitial cells stain positive. These data demonstrate a boundary interface is present in the developing cushions at early embryonic timepoints and may define a border of differentiation.