TABLE 1.
E. coli K-12 strains used
| Strain | Relevant Genotype | Source, Reference or Construction |
|---|---|---|
| ER1793 | F−
fhuA2 •(lacZ)r1 gln44e14−(McrA−) trp13-31 his-1 rpsL104 xyl-7 mtl-2 metB1 •(mcrC-mrr)114::IS10 |
New England Biolabs |
| KM21 | (recBCD)::kan | (Murphy, 1998) |
| TP539 | recG6201:: kan | (Murphy et al., 2000) |
| TP605 | sulA6209::tet | (Murphy et al., 2000) |
| TP638 | recQ6216::tet | (Murphy et al., 2000) |
| DY329 | (lacU169) nadA::Tn10, gal490, Lambda cI857, (cro-bioA) | (Yu et al., 2000) |
| CL1922* | sulA::frt-cam-frt | DY329 x linear PCR product Primers sulA-frtFOR and sulA-frtREV |
| DY351 | lacU169, gal490, cI857, (cro- bioA), D(kil-git::cam) | (Yu et al., 2000) |
| CL1016* | polB::tet | DY351 x linear PCR product Primers polBF-tetF and polBR-tetR |
| HL759 | uvrA6, zjd::Tn5 | Dr. Ann Ganesan |
| HL921 | recA::tet | (Courcelle et al., 1997) |
| HL924 | recJ284::Tn10 | (Courcelle and Hanawalt, 1999) |
| HL940 | lexA71::Tn5 | (Crowley and Hanawalt, 1998) |
| HL952 | uvrA::Tn10 | (Courcelle et al., 1999) |
| CL575 | umuC122::Tn5 | (Courcelle et al., 2005) |
| CL577 | ruvC53eda-51::Tn10 | (Donaldson et al., 2006) |
| CL578 | ruvAB6204::kan | (Donaldson et al., 2004) |
| CL579 | recF6206::tet | (Courcelle et al., 2003) |
| CL634 | dinB::kan | (Courcelle et al., 2005) |
| Strains isogenic to ER1793: | ||
| CL1312 | recJ284::Tn10 | ER1793 x P1 (HL924) |
| CL1313 | recA::tet | ER1793 x P1 (HL921) |
| CL1314 | ruvC53eda-51::Tn10 | ER1793 x P1 (CL577) |
| CL1315 | uvrA::Tn10 | ER1793 x P1 (HL952) |
| CL1316 | recQ6216::tet | ER1793 x P1 (TP638) |
| CL1317 | polB::tet | ER1793 x P1 (CL1016) |
| CL1318 | dinB::kan | ER1793 x P1 (CL634) |
| CL1319 | recF6206::tet | ER1793 x P1 (CL579) |
| CL1320 | ruvAB6204::kan | ER1793 x P1 (CL578) |
| CL1321 | recG6201:: kan | ER1793 x P1 (TP539) |
| CL1323 | umuC122::Tn5 | ER1793 x P1 (CL575) |
| CL1473 | (recBCD)::kan | ER1793 x P1 (KM21) |
| CL1605 | sulA6209::tet | ER1793 x P1 (TP605) |
| CL1623 | sulA6209::tet lexA71::Tn5 | CL1605 x P1 (HL940) |
| CL1919 | sulA::frt-cam-frt | ER1793 x P1 (CL1922) |
| CL1921 | sulA::frt-cam-frt lexA71::Tn5 | CL1919 x P1 (HL940) |
| CL1923* | sulA::frt lexA71::Tn5 | CL1921 x pCP20 (Cherepanov and Wackernagel, 1995) |
| CL 1507 | uvrA6, zjd::Tn5 | ER1793 x P1 (HL759) |
| CL 1566 | uvrA6, zjd::Tn5, recA::tet | CL1313 x P1 (HL759) |
| CL1736 | sulA::frt lexA71::Tn5 uvrA::Tn10 | CL1923 x P1 (HL952) |
*
see Materials and Methods for details; tet: tetracycline resistance; cam: chloramphenicol resistance; kan: kanamycin resistance