Figure 1.

Analysis of DNAseI sensitivity at the c-myb locus. Nuclei from both HPC7 and FMH9 cells were treated with 0 or 60 units of DNAseI and the resulting purified DNA was used as a template for Q-PCR reactions across regions of interest. The ratio between digested and undigested samples reflects the extent of hypersensitivity across regions covered by the PCR amplicons. The dashed lines indicate the basal level of digestion across the locus. Error bars represent the standard error of the mean. The defined regions of hypersensitivity and a representation of the first and second exon of the c-myb transcript are shown at the bottom of the figure.