Figure 3.

Effect of the modulation of Pbx1 and Pbx2 expression levels on c-myb mRNA. q-PCR analysis of c-myb mRNA expression was performed on HPC7 and FMH9 cells transfected with shRNA vectors for Pbx1, HoxA9 and Meis1 (a) or corresponding expression vectors (b). Similarly, vectors expressing Pbx2 shRNA (c) or Pbx2 (d) were used to modulate Pbx2 expression in both cell types alone or in combination. q-PCR results were normalized against the 18S gene and compared with the control samples transfected with scrambled shRNA. Error bars represent the standard error of the mean. Results are representative of four independent experiments. ***P<0.001; **P<0.01.