Fig. 2.

a Map of the pCCL.β-globin.PGW vector, carrying the human β-globin gene under the control of a mini-LCR as published elsewhere [45]. b, c Expression of β-globin (b) and γ-globin (c) genes following treatment of original K562 cells (white boxes) and K562-wt3 clone (black boxes) with 50 nM MTH. As evident only in the K562-wt3 clone, the β-globin gene is highly expressed. d, e Production of HbF and HbA following treatment of original K562 cells (d) and K562-wt3 clone (e) with 50 nM MTH. As evident, only in the K562-wt3 clone HbA is produced. Accumulation of globin mRNAs was analyzed by quantitative RT-PCR (for sequences of PCR primers see Table 1). Production of HbA and HbF was studied by HPLC. HbA and HbF were barely detectable in uninduced cells (data not shown)