Fig. 8.

BiP/GRP78, GADD153, Procaspase-12 and active Caspase-12 expression in PBMC from patients with chronic HCV infection following Thapsigargin or R9-CMV cell stimulation. PBMC from HCV+patients were incubated with medium alone (NS), Thapsigargin (TG) or R9-CMV for 48 h or 72 h. Data are representative of 7 similar experiments. a Protein levels as determined by immunoblotting. An immunoblot performed by stripping the membrane and reprobing it with anti-β actin polyclonal Abs is also shown. b Annexin V⁺7-AAD^- lymphocytes after 72 h of cell culture