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. Author manuscript; available in PMC: 2013 Jun 5.
Published in final edited form as: Anal Chem. 2012 May 11;84(11):4880–4885. doi: 10.1021/ac300480g

Figure 3.

Figure 3

Visualization of the m/z 58+ signal from a single NIH/3T3 fibroblast. The data was corrected in the ZcorrectorGUI and the transparency was adjusted within the GUI to allow the visualization of the sub-cellular lipid-rich feature, most likely the nuclear membrane. There are 56 images in the data set, with each image 86×86 μm2 in size and containing 256×256 pixels. (A) m/z 58+ image, data smoothed, 0.02 alpha value (a measure of transparency). (B) m/z 58+ image, only the voxels with intensity values between 1–25 are plotted, alpha value 0.04. (C) m/z 58+ image, only voxels with an intensity value of 3 are plotted, alpha value 0.25.