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. Author manuscript; available in PMC: 2013 Jun 29.
Published in final edited form as: Immunity. 2012 Jun 7;36(6):986–1002. doi: 10.1016/j.immuni.2012.05.016

Figure 2. Loss of Ly108 eliminates the SAP expression requirement for germinal center development.

Figure 2

(a-d) Wild type, Sh2d1a−/− and Slamf6−/−Sh2d1a−/− mice were infected with LCMV. Splenocytes were analyzed day 8 after infection. (a) Germinal center B cells (FashiGL7hi) and (b) plasma cells (IgDloCD138hi) are shown as a % of total B cells (CD19+CD4−). (c) Tfh cells (CXCR5+SLAMlo) and (d) GC Tfh cells (CXCR5+PD1hi) are shown as a % of activated CD4+ T cells (CD44hiCD4+). Data are representative of 3 (a-c) or 2 (d) independent experiments. N = 5 per group. (e) Wild type, Sh2d1a−/−, Slamf6−/−, and Slamf6−/−Sh2d1a−/− mice were infected with vaccinia virus. Serum antibody titers were analyzed day 8 after infection. * P < 0.05, ** P < 0.005, *** P < 0001. n.s., no statistically significant difference (P > 0.05). Error bars are SEM.