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. Author manuscript; available in PMC: 2013 Jun 29.
Published in final edited form as: Immunity. 2012 Jun 7;36(6):986–1002. doi: 10.1016/j.immuni.2012.05.016

Figure 6. Inhibition of SHP-1 reverses the adhesion defect of SAP-deficient CD4+ T cells.

Figure 6

(a-c) Activated WT, Sh2d1a−/−, Slamf6−/−, and Slamf6−/−Sh2d1a−/− SM CD4+ T cells were incubated with activated WT B cells pulsed with cognate peptide (LCMV gp66-77. 0, 0.01, or 1.0 μg/ml), in the presence or absence of SSG, followed by flow cytometry. (a) Representative flow cytometry plots, gated on CD4+ T cells. (b-c) Mean frequency of T:B conjugates in total CD4+ events, in the (b) absence or (c) presence of SSG. (d) CD4+ T cells incubated with activated Slamf6−/− B cells pulsed with cognate peptide (LCMV gp66-77. 0, 0.01, or 1.0 μg/ml). Data are shown from one of two experiments with equivalent results.