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. Author manuscript; available in PMC: 2013 Jun 29.
Published in final edited form as: Immunity. 2012 Jun 7;36(6):986–1002. doi: 10.1016/j.immuni.2012.05.016

Figure 7. Ly108 provides both positive and negative signals for NKT cell development.

Figure 7

WT, Slamf6−/−, Sh2d1a−/− and Slamf6−/−Sh2d1a−/− (‘DKO’) mice were analyzed for NKT cells. (a) Frequencies of splenic NKT cells, gated as shown in (c). (b) Absolute numbers of splenic NKT cells. (c) Representative flow cytometry plots of splenic NKT cells, gated on total CD4+ cells. Data are representative of 2 independent experiments. (d) Representative flow cytometry plots of thymic NKT cells, gated on total live CD8− cells. (e) Quantitation of (d), combining two independent experiments. (f) Developmental profiles of thymic NKT cells, gated as described in (d), from 1 of 2 representative experiments. (g) Representative flow cytometry plots of liver NKT cells, gated on total live CD19− mononuclear cells. (h) Quantitation of (g), combining two independent experiments. (i) Developmental profiles of liver NKT cells, gated as described in (g), from 1 of 2 representative experiments. ** P < 0.005, *** P < 0.0005. Error bars are SEM.