Table 1.
Primers used for qRT-PCR.
| Gene name | Accession no. | Primer sequence (5′–3′) |
|---|---|---|
| β-Actin | NM_205518 | Fa: ACGTCTCACTGGATTTCGAGCAGG |
| Rb: TGCATCCTGTCAGCAATGCCAG | ||
| TLR1-1 | AY633574 | F: CTGTCTTGCCAATCTGTC |
| R: GTGAAGGCTCCGTGTATT | ||
| TLR1-2 | NM_001098854 | F: AGCTGCAGGACTTCCTGCGC |
| R: TTGTCTGCGTCCACTGCCAC | ||
| TLR2-1 | AB050005 | F: TTAAAAGGGTGTCCAGGAG |
| R: GTCCAAACCCATGAAAGAGC | ||
| TLR2-2 | AB046533 | F: AGGCACTTGAGATGGAGCAC |
| R: CCTGTTATGGGCCAGGTTTA | ||
| TLR3 | CR407213 | F: CTGCTGCTTCCTTCGTAAGT |
| R: GCCAAACAGATTTCCAATCG | ||
| TLR4 | NM_001030693 | F: TGCACAGGACAGAACATCTCTGGA |
| R: AGCTCCTGCAGGGTATTCAAGTGT | ||
| TLR5 | CR353090 | F: CTCACCTCTCTCTCAGGGTTTT |
| R: TGGGTACACACAGTACCTGTCA | ||
| TLR7 | AJ720504 | F: CCTCGATCTCAACCCTACTTCT |
| R: CAGTATCTTTTCCTCACCACACA | ||
| TLR15 | NM_001037835 | F:GTTCTCTCTCCCAGTTTTGTAAATAGC |
| R: GTGGTTCATTGGTTGTTTTTAGGAC | ||
| TLR21 | NM_001030558 | F: AGAAGGTGTCGGAGGATGGTG |
| R:GGGCTCCAAATGCTGACTGC | ||
| MD2 | BX932484 | F: TCCATCTGGCACGCTGCTGT |
| R: GTCGTCGGTCCCGCTGCAAA | ||
| MyD88 | NM_001030962 | F: AAGTTGGGCCACGACTACCT |
| R: CAGAAAGGGTTGTTAAGCACTG | ||
| TRIF | EF025853 | F: TCAGCCATTCTCCGTCCTCTTC |
| R: GGTCAGCAGAAGGATAAGGAAAGC | ||
| TIRAP | DQ019929 | F: CTCATAGCACCACCAGCCACTC |
| R: GGGTAATCCTTCCTGTCAATGTCC | ||
| IRAK4 | AJ720408 | F: AATTGCTTGGTTTCTCAAGTG |
| R: GCAATTTCACACCTTGTGTTC | ||
| TRAF6 | CK607050 | F: AGTAAATACGAGTGCCCGATCT |
| R: TTAGCGAAGTTGTCTGGAAAAA | ||
| TRAF3 | BX935958 | F: CCAGCTCTCAGCAGCAGGAGACA |
| R: TCAGCACGAGGACACGGAAGC | ||
| IKKα | AJ720520 | F: CTTTCATCTATGGCAACTCCTG |
| R: ATGTCCAAACCAAGACGTGAT | ||
| IKKε | BU133261 | F: GTGGACGTGGTGGCCGACTG |
| R: GGCGGTTGTGTCCCCTCTGC | ||
| TAK1 | CR524033 | F: GGGCAAAGCAACTCGCCACT |
| R: TGATGTGCCTGGCCGTATTTTTCA | ||
| NF-κB2 | D16367 | F: GGTCGACGATGGCTGTGCGG |
| R: GAGGGTCGGTGTGCGTCACC | ||
| IRF7 | U20338 | F: AGACCAACTTCCGCTGCGCC |
| R: GGCATCCCCTGTGTGTGCCC | ||
| p38 | CR339030 | F: TTGGTTCCACAACTCCAGCACAG |
| R: CCGCATCCAGCACCAGCATGT |
aForward primer.
bReverse primer.