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. 2012 Jul 4;2:494. doi: 10.1038/srep00494

Figure 2. Hif-1α was necessary for survival of cancer cells in RIP1-Tag2/VKO tumors.

Figure 2

(a) Immunohistochemistry of the tumors. Red, BrdU; green, pimonidazole; blue, DAPI. Scale bar, 100μm. (b) Quantification of BrdU uptake in the overall RIP1-Tag2 lesions. Values are mean and 95-% confidence intervals. (c) Quantification of BrdU incorporation in different locations. Values are mean and 95-% confidence intervals. (d) TUNEL staining of RIP1-Tag2 tumors. Red, Apoptag; green, pimonidazole; blue, DAPI. Scale bar, 100 μm. (e) Quantification of cell death in the overall RIP1-Tag2 lesions. Values are mean and 95-% confidence intervals. (f) Quantification of cell death in locations relative to the pimonidazole-positive areas, as in (d). Values are mean and 95-% confidence intervals. Statistical analaysis was performed between the pooled areas from different genotypes.