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. 2012 May 24;107(1):63–70. doi: 10.1038/bjc.2012.188

Figure 1.

Figure 1

Hsp27 relative expression in metastatic breast tumours. (A) Expression profile of Hsp27 examined by quantitative real-time PCR by using mRNA extracted from 53 tumour biopsy specimens were obtained from patients with tumours localised to the breast (M0–N0), with only node involvement (M0–N1), and with distant metastases at diagnosis (M1) (*P⩽0.05; **P⩽0.01). TBP gene (TATAA-box-binding protein) was used as a reference here because it shows a weak variability in the breast (de Kok et al, 2005). Identical results were obtained with HBMS and HPRT housekeeping genes. (B) Hsp27 expression is given as the ratio between Hsp27 expression in each sample and the average of Hsp27 expression in the M0–N0 samples, shown by a horizontal bar in (A). Hsp27 overexpression: percentages of M0–N0, M0–N1 and M1 of samples showing a superior average as compared with the M0–N0 group average.