Figure 4.

Effect of Hsp27 depletion on bone lesion formation. Radiographic, immunostaining and histomorphometric data were obtained on day 31 after tumour cell inoculation in nude mice. (A) Osteolytic lesions on representative radiographs are indicated by white arrows. Scale bar=0.5 cm. (B) Representative bone histology of Goldner's trichrome-stained tibial metaphysis from metastatic animals. The bone is coloured in green; bone marrow and tumour cells are coloured in red. Scale bar=0.5 mm. BV/TV: bone volume relative to tissue volume. TB/STV: skeletal tumour burden relative to soft tissue volume ratio (arrows: bone decalcification) (**P⩽0.01). (C) Bone decalcification was quantified by software using Visiolab 2000, and the results are reported in the graph (**P⩽0.01).