Figure 5.

Hsp27 depletion decreases tumour formation in bone. B02-Ms27 cells and B02-Sh27 cells were injected in the left tibiae of female nude mice (**P⩽0.01). (A) Luciferase activity was quantified using the night owl technology as described in Materials and Methods. Photographs presented are representative of the results for each animal group. (B) Luciferase activity was measured weekly during 2 months and are reported on graph. (C) Bone lesions were weekly quantified by radiography of the injected tibiae. Demineralisation is depicted with white arrows. Scale bar=0.5 mm. (D) Histomorphometric data of tibiae were obtained 2 months after cell inoculation. The bone is coloured in green, whereas bone marrow and tumour cells (stars) are coloured in red. BV/TV: bone volume relative to tissue volume. TB/STV: skeletal tumour burden relative to soft tissue volume ratio. Scale bar=0.5 mm (arrows: bone decalcification). (E) Quantification of osteolysis is performed on radiographs and is presented in a graph for each group of mice.