Figure 1.

Genomic map of the EVX1 gene and its proximal promoter, and locations of methylation assays and probes. EVX1 is located at human 7p15 and has three exons. Three of the four CpG islands overlap exonic regions, and one CpG island is located within the first intron. Bisulphite sequencing was performed to assess methylation of the first two CpG islands. MethyLight probes were designed close to the transcription start site (TSS). A validation set consisted of two microarray probes, one probe located in the promoter region and one located 10 bp downstream of the TSS.