Table 1.
Primers and probes used for genotyping CYP1A1 gene polymorphisms.
| Gene/Polymorphism | Primers/Probe | Sequence |
|---|---|---|
|
| ||
| rs4646421 | Forward | CCTTCATTGATCTGACCACTCTTCA |
| Reverse | ACTCAGACTCCTTAGGGACACTTC | |
| Probe 1 (VIC)1 | CTGTCACATGTACCTCC | |
| Probe 2 (FAM) | CTGTCACATATACCTCC | |
|
| ||
| rs1799814 (Thr461Asn; m4) | Forward | GGGCAAGCGGAAGTGT |
| Reverse | GAGAAAGACCTCCCAGCGG | |
| Probe 1 (VIC) | TCGGTGAGAACATTGC | |
| Probe 2 (FAM) | CGGTGAGACCATTGC | |
|
| ||
| rs1048943(Ile462Val; m2) | Forward | GCAAGCGGAAGTGTATCGG |
| Reverse | AAGAGAAAGACCTCCCAGC | |
| Probe 1 (VIC) | TGAGACCATTGCCC | |
| Probe 2 (FAM) | TGAGACCGTTGCCC | |
1
Probes corresponding to different alleles were labeled with VIC and FAM fluorescent dyes (Applied Biosystems).
2
Polymorphic bases are underlined.