Table 1.

A-F. β-Cell viability was measured by staining cells with Trypan Blue immediately after static incubation, and is an average of counting four quadrants on a hemocytometer. Viability was assessed as percentage (%) live cells and is ± standard error, n=3. Error bars represent standard error.

A:

Treatment Live Cells (%)

Ethanol extract 25 μg/ml 77 ± 5

Ethanol extract 75 μg/ml 76 ± 4

Ethanol extract 125 μg/ml 73 ± 5

Saponin-rich fraction 25 μg/ml 94 ± 5

Saponin-rich fraction 75 μg/ml 93 ± 2

Saponin-rich fraction 125 μg/ml 93 ± 3

B:

Treatment Live Cells (%)

Compound 1 (0.005 mg/ml) 97 ± 1

Compound 1 (0.010 mg/ml) 97 ± 1

Compound 1 (0.025 mg/ml) 93 ± 3

C:

Treatment Live Cells (%)

Compound 2 (0.005 mg/ml) 96 ± 1

Compound 2 (0.010 mg/ml) 95 ± 1

Compound 2 (0.025 mg/ml) 89 ± 3*

D:

Treatment Live Cells (%)

Compound 3 (0.005 mg/ml) 98 ± 0

Compound 3 (0.010 mg/ml) 98 ± 0

Compound 3 (0.025 mg/ml) 99 ± 0

E:

Treatment Live Cells (%)

Compound 4 (0.005 mg/ml) 98 ± 0

Compound 4 (0.010 mg/ml) 99 ± 0

Compound 4 (0.025 mg/ml) 98 ± 0

F:

Treatment Live Cells (%)

Compound 5 (0.005 mg/ml) 97 ± 0

Compound 5 (0.010 mg/ml) 95 ± 1