Figure 1.
Total hematopoietic cell infiltrates in bronchoalveolar lavage fluid in response to BHT. BALB and B6 mice were injected four times weekly with BHT or with corn oil vehicle alone (n = 8 per group). Six days after the last injection, BAL fluid was collected and cells were stained for cell surface marker analysis. Live cells were selected by forward- and side-scatter profile. Populations of (a) dendritic cells (CD11b+ CD11c+) and alveolar macrophages (CD11b− CD11c+), (b) CD4 T cells (CD4+ CD90+) and CD8 T cells (CD8+ CD90+), and (c) neutrophils (Ly6G+ GR1+) were quantified. Dot plots for a representative set of animals is shown. Two-tailed statistical t-tests were performed. Error bar, standard error of the mean.