Table I.

Oligos.

Oligo	Sequence
355 arg3 ORF r2	5′-TACCAACCTTGGCATTTGCAATC-3′
356 lys1 ORF r2	5′-CAGAAGGAGCGTCTGGAGCATTG-3′
358 lys1 RT	5′-GAAGGTTTTAGGCAGTTCGAAC-3′
361 lys1 RTR	5′-TTGTTTGACTCTTAAGTCTCTCAG-3′
396 SV40p rev	5′-ATGGATATACAAGCTCCCGG-3′
397 SV40t fwd	5′-GAGATTTCGATTCCACCGCC-3′
468 HO ATG I-CeuI	5′-GATCGATAACTATAACGGTCCTAAGGTAGCGAATGCTTTCTGAAAACACGACTATTC-3′
469 HO Stop I-CeuI	5′-GATCGATCGCTACCTTAGGACCGTTATAGTTATTAGCAGATGCGCGCACCTGCGTTG-3′
470 Ipo ATG I-CeuI	5′-GATCGATAACTATAACGGTCCTAAGGTAGCGAATGGCGCTCACCAATGCTCAAATC-3′
471 Ipo Stop I-CeuI	5′-GATCGATCGCTACCTTAGGACCGTTATAGTTATTATACCACAAAGTGACTGCCCCTTTG-3′
512 ADHt fwd	5′-AGTATGAGGTCGCTCTTATTGAC-3′
516 rRNA.22F	5′-CGCAATGTGATTTCTGCCCAGTG-3′
517 rRNA.22R	5′-GTGGGAATCTCGTTAATCCATTC-3′
544 ura5 RT fwd	5′-GCTTTTTTCTTTTGTGAGTATCAACG-3′
545 ura5 RT rev	5′-GTAAATACCAACTAACTTCACTCTC-3′
611 ura5 5′fwd3	5′-TGGACTGGAGACTCACATGTCAG-3′
612 ura5 ORF rev2	5′-GCAGTTCCAGCGGTAATAACATC-3′
618 .5 ura5 up	5′-GGACAGCCATTGTAACTTATGAAC-3′
619 .5 ura5 dn	5′-GTACCATTTATTGACTGGATTAGTC-3′
620 2.0 ura5 up	5′-ATTCCATTTCCTCTGGTGAGAATG-3′
621 2.0 ura5 dn	5′-CCAATAAATGCTATAAACGAACTCC-3′
622 10.0 ura5 up	5′-AGCCTGAAAATGTGGATATTAAAGC-3′
623 10.0 ura5 dn	5′-ACAAACTCAGGTCCTAAGAAAAGC-3′
624 30.0 ura5 up	5′-AATTCCCAGTTTCCGTGTGCAAG-3′
625 30.0 ura5 dn	5′-GTAGGGTCAGATACGTAAATAGTC-3′
635 lys1 RTR2	5′-GAACAAGAGTTTTACGAGGGTCATC-3′
636 lys1 RT3	5′-ACCTTTTAGCCAAAGTGTGCGATC-3′
637 5.0 ura5 up	5′-ATCGATATCGCTCGCTGGTAGTAC-3′
638 5.0 ura5 dn	5′-ACACTAGCTGGAAATGAGCATAGTG-3′
724 rRNA.22 fwd2	5′-CTGTCTAATTAAAACATAGCATTGC-3′
725 rRNA.22 rev2	5′-CCAGGCTGTGGTTTCGCTAGATAG-3′
844 lys1 5′ fwd	5′-GAGTCATCTACAATACTGGC-3′
914 lys1 5′ I-PpoI	5′-TCGAACATTTGACACTCTCCG-3′
916 rDNA 5′ I-PpoI	5′-CAATGTGATTTCTGCCCAGTG-3′
917 rDNA 3′ I-PpoI	5′-GGCTGTGGTTTCGCTAGATAG-3′
920 his3 RT For	5′-TTACCAAGCCACTAACACCAG-3′
921 his3 RT Rev	5′-GCAGAGACCGTATACATTCCG-3′
924 I-PpoI hph 3′	5′-CCACACCCTAACTGACAAGATC-3′

Underlined sequence denotes I-CeuI sites.