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. 2012 Apr 23;9(4):341–349. doi: 10.1038/cmi.2012.13

Figure 5.

Figure 5

CD28 ligation increases the number of peritoneal cavity IFN-γ-SCs. (a) 0.5×105 and 1.0×105 C57BL/6J PerC cells±1-MA were cultured with anti-CD3±anti-CD28 as described in the ‘Methods' section. (b) 0.75×105 and 1.5×105 C57BL/6J or BALB/c PerC cells were cultured with anti-CD3±anti-CD28. (c) 1.0×105 and 1.5×105 C57BL/6J PerC cells alone or with a neutralizing anti-IL-10 mAb were cultured with anti-CD3 ±anti-CD28 costimulation. All CI values represent the ratio of the costimulated response to the uncostimulated response. *P<0.05; **P<0.005. The data shown are representative of three separate experiments that generated similar results. CI, costimulation index; IFN-γ-SC, interferon-γ-secreting cell; mAb, monoclonal antibody; PerC, peritoneal cavity.