Figure 6.
CD28 ligation increases IFN-γ-SC number for cells that exhibit a proliferative costimulatory response. (a) 1.5×105 C57BL/6J PerC or LN cells were stimulated with anti-CD3±anti-CD28 costimulation. (b) 1.0×105 C57BL/6J or IFN-γRKO PerC cells were stimulated with anti-CD3±anti-CD28 costimulation. CI values represent the ratio of the costimulated response to the uncostimulated response. *P<0.05; **P<0.005. The data shown are representative of three separate experiments that generated similar results. CI, costimulation index; IFN-γ-SC, interferon-γ-secreting cell; LN, lymph node; PerC, peritoneal cavity.