Figure 3.

Cytokine-induced regulation of MIC-1 in human prostate cancer cell line (LNCaP cells). (A) RT-PCR analysis shows the expression of the MIC-1 gene on LNCaP cells treated with various cytokines. GAPDH was used as an internal control. (B) Differential secretion of the MIC-1 protein modulated by inflammatory cytokines. As an internal control, β-actin was used on the lysates from the same set of cells demonstrating the secretion of MIC-1 (lower panel). RPMI-1640 phenol red-free, serum-free medium served as a control for cytokine treatment, while the medium containing 5% FBS served as a positive control.