Table 2.
Effect of PMA on the intracellular and extracellular IL-1α production and determination of cytotoxic effects.
| PMA conc. (ng/mL) | Ctrl. | 6.25 | 12.5 | 25 | 50 | 100 |
|---|---|---|---|---|---|---|
| IL-1α concentration (pg/mL) | ||||||
| Intracellular | 256.30 ± 26.24a A | 266.71 ± 25.06a A | 223.29 ± 82.14a A | 200.91 ± 69.97a A | 234.73 ± 31.16a A | 233.17 ± 36.38a A |
| Extracellular | 1.74 ± 0.23a B | 2.60 ± 0.87a B | 1.78 ± 0.696a B | 2.28 ± 0.81a B | 1.86 ± 0.50a B | 1.93 ± 0.40a B |
| % LDH release | 7.12 ± 0.93a | 7.90 ± 1.65a | 6.91 ± 0.68a | 9.48 ± 2.67a | 6.52 ± 0.65a | 7.22 ± 0.69a |
Cells were seeded at a density of 30 × 104/well and incubated for 6 h. Values represent the mean ± standard deviation of 6 replicates. Cytotoxicity represents LDH release as a percentage of the total. Letters in superscript indicated significant differences between control and PMA concentration; letters in subscript indicated significant differences between intracellular and extracellular IL-1α production. P < 0.05 was considered significant. PMA: phorbol 12-myristate 13-acetate; LDH: lactate dehydrogenase.