Table 1.
Characterization in silico of a panel of 54 recombinant proteins, included in the present study.
| Antigen (ORF no.) | Predicted localization | Theoretical MW | Homology with other mycobacteria |
|---|---|---|---|
| MAP 0011 | Cytoplasmic | 19,196 kDa | Iron-regulated peptidyl-prolyl cis-trans isomerase A in Mycobacterium tuberculosis H37Rv |
| MAP 0034 | Cytoplasmic-Membrane | 44 kDa | P44 protein in Mycobacterium avium subsp. avium |
| MAP 0047c | Extracellular | 41,1 kDa | Lpp-LpqN family conserved in Mycobacteriaceae |
| MAP 0038 | Unknown | 48,7 kDa | Hypothetical protein Mb0027 in Mycobacterium bovis AF2122/97 |
| MAP 0187 | Extracellular | 23 kDa | Superoxide dismutase in Mycobacterium bovis |
| MAP 0209c | Extracellular | 56,5 kDa | Protein potentially involved in peptidoglycan biosynthesis in Mycobacterium avium subsp. avium |
| MAP 0210c | Cytoplasmic-Membrane | 30,7 kDa | Secreted antigen P36/P34 precursor in Mycobacterium bovis , maxima ident 60% |
| MAP 0211 | Cytoplasmic | 46 kDa | UDP-galactopyranose mutase glf in Mycobacterium tuberculosis |
| MAP 0297 | Cytoplasmic | 55,23 kDa | Hypothetical protein Mb1161 Mycobacterium bovis AF2122/97 |
| MAP 0334 | Unknown | 34,386 kDa | Oxidoreductase in Mycobacterium bovis AF2122/97 |
| MAP 0900 | Cytoplasmic-Membrane | 29,6–34 kDa | Antigen 34 kDa in Mycobacterium tuberculosis CDC1551 |
| MAP 0946c | Cytoplasmic-Membrane | 33,5 kDa | Sigma factor in Mycobacterium avium 104 |
| MAP 1012c | Cytoplasmic | 37,374 kDa | Hypothetical protein TMAG_01006 in Mycobacterium tuberculosis |
| MAP 1050c | Cytoplasmic | 33 kDa | Peptidyl-prolyl cis-trans isomerase, cyclophilin-type conserved in Mycobacterium |
| MAP 1272 | Extracellular | 33,4 kDa | NLP/P60 family protein in Mycobacterium tuberculosis CDC1551 |
| MAP 1293 | Unknown | 49,24 kDa | Histidinol dehydrogenase his D in Mycobacterium tuberculosis |
| MAP 1308 | Unknown (This protein may have multiple localization sites.) | 46 kDa | Prolipoprotein diacylglyceryl transferase lgt in Mycobacterium tuberculosis |
| MAP 1564c | Unknown | 23,01 kDa | Short chain dehydrogenase in Mycobacterium bovis |
| MAP 1589c | Cytoplasmic | 21,60 KDa | Alkyl hydroperoxide reductase subunit C in Mycobacterium tuberculosis |
| MAP 1653 | Unknown | 16,7–20 kDa | Thiol peroxidase tpx in Mycobacterium tuberculosis T17 |
| MAP 1693c | Unknown | 18,30 kDa | Peptidyl-prolyl cis-trans isomerase domain-containing protein conserved in Mycobacterium |
| MAP 1754c | Cytoplasmic-Membrane | 30,84 kDa | Hypothetical protein Rv2005c in Mycobacterium tuberculosis H37Rv |
| MAP 1889c | Cytoplasmic | 28 kDa | Wag31 protein in Mycobacterium avium 104 |
| MAP 1962 | Cytoplasmic | 53,68 kDa | Glutamine synthetase glnA1 in Mycobacterium tuberculosis |
| MAP 2020 | Unknown | 26,90 kDa | Cutinase in Mycobacterium bovis AF2122/97 |
| MAP 2167 | Extracellular | 17 kDa | Low molecular weight protein antigen cfp2 in Mycobacterium tuberculosis |
| MAP 2182 | Cytoplasmic-Membrane | 16 kDa | Deazaflavin-dependent nitroreductase family protein in Mycobacterium |
| MAP 2513 | Cytoplasmic | 36,50 kDa | Alkanal monooxygenase alpha chain in Mycobacterium avium 104 |
| MAP 2609 | Cytoplasmic-Membrane | 11,40 kDa | Low molecular weight T-cell antigen TB8.4 in Mycobacterium tuberculosis |
| MAP 2676c | Cytoplasmic | 13,89 kDa | Hypothetical protein MAV_1246 in Mycobacterium avium 104 |
| MAP 2685 | Unknown | 21,20 kDa | Hypothetical protein BCG_1169c in Mycobacterium bovis BCG str. Pasteur 1173P2 |
| MAP 2878c | Cytoplasmic | 25,43 kDa | Dihydrodipicolinate reductase in Mycobacterium bovis BCG str. Pasteur 1173P2 |
| MAP 2942c | Extracellular | 18,30 kDa | Soluble secreted antigen MPT53 in Mycobacterium tuberculosis |
| MAP 2956 | Cytoplasmic | 30,02 kDa | 30S ribosomal protein S2 in Mycobacterium tuberculosis H37Rv |
| MAP 3175c | Cytoplasmic | 41,38 kDa | Peptide chain release factor 2 in Mycobacterium tuberculosis H37Rv |
| MAP 3194 | Cytoplasmic | 30,46 kDa | Pyruvate carboxyl transferase in Mycobacterium avium |
| MAP 3205 | Cytoplasmic | 27 kDa | nuoE NADH dehydrogenase subunit E in Mycobacterium avium 104 |
| MAP 3206 | Cytoplasmic | 48-49 kDa | nuoF NADH-Quinone oxidoreductase subunit F in Mycobacterium tuberculosis |
| Mb 3341c | Unknown | 10,63 kDa | Equivalent to Rv3312A, len: 103 aa, from Mycobacterium tuberculosis strain H37Rv |
| MAP 3402 | Cytoplasmic | 33,28 kDa | Thiosulfate sulfurtransferase in Mycobacterium bovis AF2122/97 |
| MAP 3457 | Cytoplasmic | 47,61 kDa | O-acetylhomoserine sulfhydrylase metC in Mycobacterium tuberculosis T92 |
| MAP 3491 | Cytoplasmic | 28,16 kDa | Hydrolase in Mycobacterium tuberculosis SUMu003 |
| MAP 3527 | Unknown | 35,70 kDa | Serine protease PepA in Mycobacterium tuberculosis H37Rv |
| MAP 3627 | Cytoplasmic | 37,37 kDa | O-methyltransferase in Mycobacterium tuberculosis H37Rv |
| MAP 3651 | Cytoplasmic | 44 kDa | Acyl-CoA dehydrogenase fadE3 in Mycobacterium bovis BCG |
| MAP 3692 | Cytoplasmic | 47 kDa | fabG 3-ketoacyl-ACP reductase in Mycobacterium tuberculosis H37Rv |
| MAP 3840 | Cytoplasmic | 67 kDa | Heat shock protein 70, molecular chaperone DnaK in Mycobacterium |
| MAP 3841 | Unknown | 23,57 kDa | Heat shock protein GrpE in Mycobacterium bovis AF2122/97 |
| MAP 3857 | Cytoplasmic | 18,73 kDa | Orotate phosphoribosyltransferase phosphoribosyltransferase in Mycobacterium tuberculosis CDC1551 |
| MAP 3936 | Cytoplasmic | 57 kDa | Heat shock protein 65, GroEL in Mycobacterium sp |
| MAP 4000c | Unknown | 12 kDa | Esat-6 like protein esxF in Mycobacterium and hypothetical protein Mb3935c in Mycobacterium bovis |
| MAP 4143 | Cytoplasmic | 43,77 kDa | Iron-regulated elongation factor tu in Mycobacterium tuberculosis |
| MAP 4147 | Cytoplasmic | 42,09 kDa | Ferredoxin reductase in Mycobacterium tuberculosis H37Rv |
| MAP 4227c | Cytoplasmic | 30,14 kDa | Hypothetical protein Rv3463 in Mycobacterium tuberculosis H37Rv |