Table 2.
Spot intensities for protein macroarrays.
| Antigen assayed | Mean spot intensity of proteins exposed to sera | ||
|---|---|---|---|
| control (n = 10) |
PTB infected (n = 25) |
TB infected (n = 8) |
|
| MAP 0011 | 112 | 135 | 0 |
| MAP 0034 | 240 | 247 | 0 |
| MAP 0038 | 141 | 860 | 0 |
| MAP 0047 | 344 | 309 | 0 |
| MAP 0187c | 184 | 0 | 0 |
| MAP 0209c | 367 | 499 | 0 |
| MAP 0210c | 0 | 398 | 0 |
| MAP 0211 | 0 | 572 | 0 |
| MAP 0297 | 117 | 225 | 130 |
| MAP 0334 | 108 | 167 | 1 |
| MAP 0900 | 0 | 749 | 0 |
| MAP 0946 | 218 | 900 | 295 |
| MAP 1012 | 280 | 0 | 0 |
| MAP 1050 | 268 | 275 | 0 |
| MAP 1272 | 223 | 733 | 0 |
| MAP 1293 | 585 | 481 | 396 |
| MAP 1308 | 163 | 894 | 0 |
| MAP 1564 | 259 | 344 | 0 |
| MAP 1589c | 323 | 682 | 466 |
| MAP 1653 | 398 | 844 | 461 |
| MAP 1693 | 226 | 967 | 209 |
| MAP 1754 | 0 | 142 | 27 |
| MAP 1889c | 280 | 180 | 494 |
| MAP1962 | 341 | 270 | 354 |
| MAP 2020 | 0 | 150 | 228 |
| MAP 2167 | 315 | 0 | 0 |
| MAP 2182c | 223 | 117 | 192 |
| MAP 2513 | 41 | 164 | 291 |
| MAP 2609 | 137 | 308 | 0 |
| MAP 2676 | 201 | 138 | 0 |
| MAP 2685 | 317 | 323 | 0 |
| MAP 2878 | 307 | 323 | 0 |
| MAP 2942 | 136 | 251 | 0 |
| MAP 2956 | 345 | 119 | 424 |
| MAP 3175 | 343 | 130 | 0 |
| MAP 3194 | 0 | 100 | 0 |
| MAP 3205 | 0 | 0 | 0 |
| MAP 3206 | 100 | 200 | 0 |
| Mb 3341 | 159 | 171 | 178 |
| MAP 3402 | 205 | 178 | 250 |
| MAP 3457 | 447 | 365 | 325 |
| MAP3491 | 286 | 454 | 177 |
| MAP 3527 | 528 | 414 | 635 |
| MAP 3627 | 207 | 265 | 0 |
| MAP 3651 | 1000 | 906 | 958 |
| MAP 3692c | 305 | 212 | 0 |
| MAP 3840 | 0 | 198 | 712 |
| MAP 3841 | 707 | 678 | 457 |
| MAP 3857 | 373 | 267 | 265 |
| MAP 3936 | 381 | 544 | 691 |
| MAP 4000c | 134 | 242 | 0 |
| MAP4143 | 413 | 397 | 439 |
| MAP 4147 | 1000 | 556 | 0 |
| MAP 4227 | 138 | 101 | 0 |
| PPDA | 208 | 105 | 256 |
| PPDB | 346 | 531 | 1468 |
| PPA-3 | 131 | 609 | 108 |
| Cocktail | 140 | 1460 | 0 |
Intensities were obtained using ImageQuant TL Array Version 7.0 Software, (GE Healthcare, Pittsburgh, PA). Antigens selected to evaluate as cocktail are shown in bold. PPA-3: Paratuberculosis protoplasmatic antigen (Allied Monitor, Inc. USA); PPDA and PPDB: avian- and bovine-derivative protein purified, respectively (Prionic Switzerland). Cocktail: mix of the 7 antigens shown in bold.