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. Author manuscript; available in PMC: 2012 Jul 5.
Published in final edited form as: Oncogene. 2010 Sep 6;29(48):6367–6377. doi: 10.1038/onc.2010.364

Figure 4.

Figure 4

h7C10 activity involves AKT signaling. (a) RMS cells were treated with h7C10 for 24 h. The cell lysates were analyzed with an IGF1R sandwich immunoassay. (b) Immunoblots against t/p-ERK, t/p-AKT and AKT downstream targets with cells treated with h7C10 at the indicated time points. (c) Cells were infected with myr-AKT or control adenovirus for 2 days, followed by h7C10 treatment for 4 h. Immunoblots revealed the restoration of AKT signaling with myr-AKT. (d) myr-AKT adenovirus infected cells were treated with h7C10 for 2 days and analyzed for viable cells. Statistic interaction P-values between h7C10 and myr-AKT were determined using two-way ANOVA.