Figure 4 .

Reintegration of Ds elements in yeast. (A) Examples for reintegrated Ds elements from AcTPase_4x-promoted transposition. DNA gel blot with PvuI-digested genomic DNA from yeast strains BY4723, CWY1, and nine individual Ade⁺ revertants, hybridized with a Ds-specific probe. The gray arrowhead marks the Ds donor site in the ade2 locus of yeast strain CWY1. (B) Distribution of Ds insertion sites in the yeast genome. The 16 yeast chromosomes are drawn to scale as gray bars with the centromere for each chromosome indicated by a dot. The gray triangle marks the initial position of the Ds element in the ade2 locus on yeast chromosome XV. Red triangles indicate reintegration of the Ds element promoted by the hyperactive AcTPase_4X, and open triangles by the AcTPase. Scale bar is in kilobase pairs.