Table 1 . Ds excision in Arabidopsis.
Ds excision sitea | |||||||
---|---|---|---|---|---|---|---|
Transposase | Lineb | Plantsc | Excisionsd | A | B | C | D |
AcTPase | 30 | 54 | — | — | — | — | — |
31 | 50 | — | — | — | — | — | |
20 | 66 | 10 | 6 | 4 | — | — | |
Σe | 170 | 10 | 6 | 4 | — | — | |
AcTPase4x | 29 | 68 | 21 | 7 | 5 | 6 | 3 |
42 | 72 | 21 | 13 | — | 5 | 3 | |
41 | 72 | 28 | 17 | 3 | 4 | 4 | |
Σ | 212 | 70 | 37 | 8 | 15 | 10 |
Classification of individual plants according to the Ds excision sites isolated. A: number of plants with “gc” footprint. B: number of plants showing unique footprints including “restoring” excision products. C: number of plants with multiple footprints. D: sequence was unreadable distal to the Ds excision site.
The three independent plant lines analyzed for each transposase are, with the exception of 29 (Ds5), all derived from Ds starter line Ds24.
Number of plants analyzed for excision of Ds.
Number of individual plants with empty Ds donor sites.
Σ = total number of AcTPase and AcTPase4x plants and excisions, respectively.