Table 1.
Male pronuclear migration distance at pronuclear meetinga
| Genotype | Rangeb [% egg length] | Number of embryos (% of n)
|
|||
|---|---|---|---|---|---|
| Decreased [<14%] | wt [14-20%] | Increased [>20%] | |||
| wt [n=16] | 14-20 | 0 | 16 (100%) | 0 | |
|
| |||||
| WAVE/SCAR | wve-1(ne350) [n=14] | 0-16 | 10 (71%) | 4 (29%) | 0 |
| gex-3(zu196) [n=12] | 0-21 | 5 (42%) | 7 (58%) | 0 | |
| gex-3(RNAi) [n=14] | 0-9 | 14 (100%) | 0 | 0 | |
| arp-2(RNAi) [n=15] | 0-7 | 15 (100%) | 0 | 0 | |
|
| |||||
| SUNKASH | unc-84(e1410) [n=17] | 0-19 | 10 (59%) | 7 (41%) | 0 |
| unc-84(RNAi) [n=15] | 0-23 | 8 (53%) | 7 (47%) | 0 | |
| unc-83(ku18) [n=15] | 10-22 | 2 (13%) | 11 (73%) | 2 (13%) | |
| unc-83(RNAi) [n=11] | 13-21 | 1 (9%) | 8 (73%) | 2 (18%) | |
| anc-1(e1873) [n=12] | 13-22 | 2 (17%) | 9 (75%) | 1 (8%) | |
| kdp-1(RNAi) [n=17] | 7-24 | 7 (41%) | 9 (53%) | 1 (6%) | |
|
| |||||
| Double mutants | wve-1(ne350); kdp-1(RNAi) [n=16] | 1-18 | 12 (75%) | 4 (25%) | 0 |
| gex-3(zu196); kdp-1(RNAi) [n=16] | 1-17 | 10 (62%) | 6 (38%) | 0 | |
| gex-3(RNAi); unc-84(e1410) [n=10] | 0-9 | 10 (100%) | 0 | 0 | |
| arp-2(RNAi); unc-84(e1410) [n=9] | 0-19 | 8 (89%) | 1 (11%) | 0 | |
| kdp-1(RNAi); unc-84(e1410) [n=15] | 3-17 | 12 (80%) | 3 (20%) | 0 | |
| gex-3(RNAi); unc-83(ku18) [n=10] | 9-21 | 2 (20%) | 8 (80%) | 0 | |
| unc-83(ku18); arp-2(RNAi) [n=8] | 8-18 | 4 (50%) | 4 (50%) | 0 | |
| kdp-1(RNAi); unc-83(ku18) [n=16] | 4-23 | 5 (31%) | 10 (63%) | 1 (6%) | |
| anc-1(e1873); unc-83(RNAi) [n=11] | 12-18 | 3 (27%) | 8 (73%) | 0 | |
| anc-1(e1873); kdp-1(RNAi) [n=14] | 4-16 | 12 (86%) | 2 (14%) | 0 | |
|
| |||||
| par-2(RNAi) [n=16] | 0-33 | 2 (12%) | 2 (13%) | 12 (75%) | |
a
Male pronuclear migration distance indicates distance between posterior plasma membrane and posterior membrane of the male pronucleus relative to total egg length [%]
b
Range reflects male pronuclear migration distance relative to total egg length [%] for each genotype