Figure 5. .

Vitamin E pretreatment favors autofluorescence recovery following bleaching. Total fluorescence and absorbance were determined from total chromatographic peak area (430 nm monitoring) in non-irradiated (control) A2E-containing cells and A2E-containing cells analyzed 1, 8, and 24 hours after irradiation. Cells were allowed to accumulate vitamin E (vit E) or vitamin E acetate (vit E acet) 24 hours before irradiation (6 minutes) or were not pre-treated (no vit E). Mean ± SEM, three experiments.