Figure 6. Positive gene expression from pDNA delivered as a liquid formulation via microneedles to viable human skin.
(A) En face image showing positive points of gene expression resulting from multiple applications of the microneedle device through a pre-applied solution of plasmid encoding β-galactosidase and 24 hrs in organ culture (bar = 1mm). (B) A human epidermal sheet treated with a microneedles and a liquid formulation of plasmid encoding EGFP (bar = 50μm). (C) 10μm histological section showing positive gene confined to the epidermis (bar = 100μm).