Table 1. Oligonucleotides used for the amplification.
| Rat Target | Sequence | Sizea (bp) | AnnTb (°C) | Cycles | |
| CRLR | Sense | 5′-CAACTGCTGGATCAGCTCAG-3′ | 249 | 60 | 35 |
| Antisense | 5′-CATCGCTGATTGTTGACACC-3′ | ||||
| RAMP1 | Sense | 5′-CAATCCGGAAGTGGACAAGTT-3′ | 197 | 60 | 31 |
| Antisense | 5′-GACACCTACACGATGCCCTCT-3′ | ||||
| RAMP2 | Sense | 5′-CAAGGACTGGTGCAACTGGAC-3′ | 257 | 60 | 31 |
| Antisense | 5′-ACACCACAAGCGTAACGAGGA-3′ | ||||
| RAMP3 | Sense | 5′-ACCTGTCGGAGTTCATCGTGT-3′ | 225 | 58 | 33 |
| Antisense | 5′-TAGCCACGGTCAACAAGACTG-3′ | ||||
| β-actin | Sense | 5′-TCCTGGGTATGGAATCCTGTG-3′ | 291 | 58 | 25 |
| Antisense | 5′-CTCAGGAGGAGCAATGATCTTG-3′ | ||||
| ANP | Sense | 5′-TCGAGCAGATCGCAAAAGATC-3′ | 150 | 60 | 25 |
| Antisense | 5′-CACACTAAACCACTCATCTAC-3′ | ||||
| BNP | Sense | 5′-TCTGCTCCTGCTTTTCCTTA-3′ | 256 | 60 | 26 |
| Antisense | 5′-GAACTATGTGCCATCTTGGA-3′ | ||||
aExpected sizes of the amplicons; bAnnT: annealing temperature used for the PCR reaction. CRLR: Calcitonin receptor-like receptor; RAMP: Receptor activity-modifying proteins; ANP: atrial natriuretic peptide; BNP: brain natriuretic peptide.