Figure 1. Atp7b inactivation induces age-dependent changes in urine copper content.

(A) Urinary copper concentration and (B) total amount from wild-type (WT) and Atp7b ^−/− (KO) mice at different ages. Urine collected over 24 hours and measured by atomic absorption. Data reported as mean concentration ± SD, n = 3 to 13, animals per age group. *P<0.05 versus age-matched wild type. Boxplot middle horizontal bar represents the median; the dotted horizontal line signifies the mean. The while upper and lower box values signify the 75^th and 25^th percentiles, whiskers represent the 10^th and 90^th percentiles. The black dots represent outliers (three SD from the mean).