Figure 3. PET-CT analysis of ⁶⁴Cu distribution in live mice at 24 hrs after oral administration of ⁶⁴CuCl₂.

Representative PET-CT images of (A) Atp7b ^−/− and (B) wild-type mice at 24 hours post oral administration (PO) of ⁶⁴CuCl₂. Orange-brown color denotes ⁶⁴Cu radioactivity. Red arrows and white arrows identify liver and gastrointestinal tract ⁶⁴Cu radioactivity, respectively. (C) Hepatic ⁶⁴Cu radioactivity in Atp7b^−/− and wild-type mice at 24 hrs PO (p<0.001). Age-dependent ⁶⁴Cu radioactivity in the (D) kidneys (p = 0.38 for group effect) and (E) urinary bladder of wild-type and Atp7b ^−/− mice at 24 hr PO. %ID/g, percentage of administration dose per gram. Data presented as mean ± SD. N = 5, number of mice with same age and genotype. Additional data and details in Figure S2 and Information S1.