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. Author manuscript; available in PMC: 2012 Jul 5.
Published in final edited form as: Biochemistry. 2011 Apr 13;50(18):3777–3783. doi: 10.1021/bi101404n

Table 4.

Summary of ion flow and DNA translocation behavior.

System Simulated open pore current(pA) 1 Extrapolated open pore current at600 mV (pA) 2 Simulated residual ion count 3 Extrapolated partial current(pA) (13)4 DNA translocation in 20 ns5
M113D 489±9 567 n/a n/a None
E111N 824±53 694 114±12 76.34 Full
WT 680±53 634 80±16 69.74 Partial
M113R 580±38 709 17±2 28.36 Partial
T117R 519±13 572 24±14 17.16 Partial
N123R 427±32 390 23±6 11.7 Partial
G119R 460±22 562 15±9 11.24 Full
N121R 439±8 486 14±20 14.58 Partial
T115R 521±14 618 7±6 12.36 Partial
1

Simulated currents in the absence of DNA, calculated as in (18).

2

Extrapolated from experimental IV curves of the open pore at +120 mV in (13).

3

Ion flux in the presence of DNA (i.e. residual), once two DNA bases had crossed the K147 constriction.

4

Calculated from experimental percentage block in (13) applied to the extrapolated currents from the IV curve.

5

Observed translocation behavior included “none” (no entry into the pore), “partial” (DNA strand does not completely translocate through the pore in 20 ns) or “full” (the strand both enters the pore and exits on the trans side).