Figure 1. .

Carcinine forms an adduct with 4-HNE in vitro. Carcinine (0.5 mM) was incubated with 0 or 0.64 mM 4-HNE for 16 hours at room temperature, and samples were analyzed by HPLC/MS. (A) Representative chromatogram and corresponding mass spectrum obtained from carcinine incubated with 0 mM 4-HNE. (B) Representative chromatogram and corresponding mass spectrum obtained from carcinine incubated with 0.64 mM 4-HNE. Carcinine gives a peak at 318.1 m/z and 4-HNE-carcinine adduct at 474.2 m/z. This experiment was repeated three times with similar results.