Figure 3. .

Carcinine reverses 4-HNE modification of retinal proteins. Preformed 4-HNE-protein adducts were incubated for indicated times with 0 (−) or 1 mg (+) carcinine before dot-blot analysis. A representative dot-blot showing time-course of reversion is shown. Signals of the upper blot were quantified, normalized to protein amount (lower blot), and plotted as percentage of adducts remaining. Results from three experiments, each done in triplicate, were quantified and plotted. Error bars correspond to SEM.