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Determination of extinction coefficient at 380 nm for the retinal chromophore in the rhodopsin/transducin complex. Spectrum 1 is that of the purified complex in 5 mM HEPES buffer, pH 7.5, containing 0.1 mM MgCl2 and 0.02% (w/v) DDM. Spectrum 2 is that of the same sample immediately following addition of 50 mM sodium phosphate buffer, pH 3.5, and 0.5% SDS (final concentrations). Spectrum 2 has been corrected for dilution.
