Fig. 3.

Stokes radius determinations by gel filtration and DLS. (A) Gel filtration profile of α_IIbβ₃ headpiece alone or bound with antagonists. The untagged α_IIbβ₃ headpiece was mixed with near-saturating concentrations of RUC-1, RUC-2, or tirofiban and incubated at room temperature for 1 hour before chromatography on Superdex 200 in tris-buffered saline plus 1 mM Ca²⁺/Mg²⁺. The elution volumes are shown in parentheses. (B) Stokes radii calculated from gel filtration or DLS. Data are means ± SD (n = 2 for gel filtration; n =3forDLS).