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. Author manuscript; available in PMC: 2012 Jul 5.
Published in final edited form as: Sci Transl Med. 2012 Mar 14;4(125):125ra32. doi: 10.1126/scitranslmed.3003576

Fig. 4.

Fig. 4

Effect of RUC-2 on priming platelets to bind fibrinogen. Washed platelets were incubated with eptifibatide (1 μM), tirofiban (0.5 μM), RGDS (100 μM), RUC-1 (100 μM), or RUC-2 (1 μM) for 20 min and fixed with 1% paraformaldehyde for 40 min. After the paraformaldehyde was quenched with glycine (5 mM), platelets were washed and incubated with fluorescent fibrinogen (200 μg/ml) in the presence of 2 mM Ca2+ and 1 mM Mg2+. After washing, the platelets were analyzed by flow cytometry. (A) Mean ± SD (n = 4) net platelet fluorescence (NPF), defined as the percentage of platelets with fluorescence intensity values above 25 arbitrary units (AU) in the presence of one of the antagonists minus the percentage in the absence of the antagonist. Eptifibatide blocked the binding of fibrinogen induced by the antagonists, yielding values equal to or below the control value. (B) Fluorescence data from one of the four similar experiments.