Figure. 4. Determination of EC50 of select novologues.
A) DRG sensory neurons were incubated in the absence or presence of 0.1–1000 nM of the indicated novologue overnight and then subjected to 4 hrs of hyperglycemia. Cell viability was measured as described in Experimental Methods and the data expressed as percent of normoglycemic controls. Under hyperglycemic conditions and in the absence of any novologues, cell viability was 20% ± 7. B) The EC50 was determined using the ECanything function of GraphPad Prism 5.0 and the mean ± SEM (n=3–8) is shown. #, p< 0.05 versus compound 2.