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. 2012 Jun 27;32(26):8940–8951. doi: 10.1523/JNEUROSCI.0026-12.2012

Figure 4.

Figure 4.

Blocking LDH does not affect the [O2] and [NADH] decreases. A, Effect of LDH inhibition on lactate level in the extracellular solution (5 slices). Oxamate significantly inhibits lactate accumulation (the inhibition of LDH is even stronger than the reduction in lactate concentration shown in the graph, because lactate made before the oxamate was applied will not be removed by the oxamate). B, Experimental protocol. Oxamate was added or not added during stage 2, and responses in E–G were expressed as a percentage of those in stage 1. C, D, Example traces showing the effect of 20 mm oxamate on the O2 transient (C) and the NADH dip and overshoot produced by Schaffer collateral stimulation (D). E–G, Mean data showing the effect of oxamate on the [O2] decrease (E), initial NADH fluorescence decrease (F), and late NADH fluorescence increase (G), normalized as in B. Solutions contained 95% O2.